![]() The markers flanking the QTL for omega-3 fatty acids can be used in MAS to accelerate the genetic improvements of these traits in salt-tolerant tilapia. Our data suggest that the total lipid and omega-3 fatty acid content were determined by multiple genes in tilapia. QTL for omega-3 fatty acids were located on four LGs (LG11, -18, -19, and -20) and explained 5.0 to 7.5% of the phenotypic variance. Significant and suggestive QTL for total lipid content were mapped on six linkage groups (LG3, -4, -6, -8, -13, and -15) and explained 5.8-8.3% of the phenotypic variance. The linkage map was 2076.7 cM long and consisted of 22 linkage groups. We constructed a linkage map with 784 markers (151 microsatellites and 633 SNPs). Genotyping by sequencing (GBS) technology was used to discover and genotype SNP markers, and microsatellites were also genotyped. The total omega-3 content in F 2 hybrid tilapia was 2.5 ± 1.0 mg/g, higher than that (2.00 mg/g) in freshwater tilapia. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F 2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Tilapia is one of most important foodfish species. Lin, Grace Wang, Le Ngoh, Si Te Ji, Lianghui Orbán, Laszlo Yue, Gen Hua Mapping QTL for Omega-3 Content in Hybrid Saline Tilapia. Fish of average weight 12.59g were stocked at a density of 20 fish m–³ and were fed a 30% . guineensis (female) — was evaluated for 233 days. , on the growth rate, feeding efficiency and mortality rates of hybrid tilapia — Tilapia zillii (male) x T. Comparative analysis of the aquaculture potential of hybrid Tilapia.
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